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Todaro et al., Virology, 27: 27 (1965) [PMID: 4284655] CABRI:DSMZ_MUTZ Accession_number ACC 202 Cell_line_name 3T6 Brief_description confirmed as mouse with IEF of AST, G6PD, MDH, NP and with species-PCR established from disaggregated Swiss albino mouse embryos in 1963; cells were described to secrete collagen and hyaluronic acid Description Swiss albino mouse fibroblasts established from disaggregated Swiss albino mouse embryos in 1963; cells were described to secrete collagen and hyaluronic acid confirmed as mouse with IEF of AST, G6PD, MDH, NP and with species-PCR Depositor Dr. H. G. Drexler, DSMZ, Braunschweig, Germany Morphology fibroblasts growing adherently as monolayer Culture_conditions 85-90% Dulbecco's MEM + 10-15% FBS split confluent culture ca. 1:10 every 2-3 days using trypsin/EDTA, seed out about 2 x 106 cells/175 cm 2 ; cells may clump after trypsinization and may be difficult to disperse at 37C with 5-10% CO2 cell harvest of about 15 x 106 cells/175 cm 2 Viruses ELISA: reverse transcriptase positive; PCR: SMRV - Properties Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule Doubling_time ca. 15 hours Mycoplasma negative in DAPI, microbiological culture, RNA hybridization assays Cytogenetics murine flat-moded hypertriploid/hypotetraploid karyotype - 67(64-73)<3n> Bibliographic_reference Todaro et al., J Cell Biol, 17: 27 (1963) [PMID: 13985244 ]

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